Conclusion
L'objectif de ce travail était l'optimisation des
paramètres d'hydrolyse de son de blé dans le but de l'utiliser
comme milieu de culture naturel de C. glutamicum 2262 pour la
production glutamtique.
La première partie de ce travail a essentiellement
été basée sur l'optimisation des paramètres
d'hydrolyse de son de blé en utilisant le model statistique Box Behnken.
Nous avons montré que les valeurs optimales de l'hydrolyse sont les
suivants : 85°C pour la température d'hydrolyse, 68 min pour le
temps d'hydrolyse et 11% pour la concentration d'acide sulfurique.
Dans la deuxième partie on a démontré que
l'hydrolysat de son de blé contient les différents
éléments nutritionnels (source de carbone, source d'azote et sels
minéraux) qui peuvent être utilisés par C. glutamicum
2262 afin de produire l'acide glutamique.
Finalement, l'objectif du travail est atteint. Cependant de
nombreuses perspectives peuvent être envisagées :
- L'enrichissement de milieu de culture par d'autres sources
d'azote et de vitamine.
- Utilisation de model Plakett Burmen pour étudier
l'effet des éléments nutritionnels sur la production
glutamique.
- Utilisation le mode semi-continu ou le mode continu pour une
meilleure productivité.
- Valorisation des autres parties de blé tel que la paille
de blé.
- Utilisation des enzymes spécifiques capables
d'augmenter le rendement de l'hydrolyse de cellulose.
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