Annex
Tables de Mac Grady
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2 tubes par dilution
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3 tubes par dilution
|
|
Nombre caractéristique
|
Nombre de cellules
|
Nombre caractéristique
|
Nombre de cellules
|
Nombre caractéristique
|
Nombre de cellules
|
Nombre caractéristique
|
Nombre de cellules
|
|
000
001
010
011
020
100
101
110
111
120
121
200
201
210
211
212
220
221
222
|
0.0
0.5
0.5
0.9
0.9
0.6
1.2
1.3
2.0
2.0
3.0
2.5
5.0
6.0
13.0
20.0
25.0
70.0
110.0
|
000
001
010
011
020
100
101
102
110
111
120
121
130
200
|
0.0
0.3
0.3
0.6
0.6
0.4
0.7
1.1
0.7
1.1
1.1
1.5
1.6
0.9
|
201
202
210
211
212
220
221
222
223
230
231
232
300
301
|
1.4
2.0
1.5
2.0
3.0
2.0
3.0
3.5
4.0
3.0
3.5
4.0
2.5
4.0
|
302
310
311
312
313
320
321
322
323
330
331
332
333
|
6.5
4.5
7.5
11.5
16.0
9.5
15.0
20.0
30.0
25.0
45.0
110.0
140.0
|
|
5 tubes par dilution
|
|
Nombre caractéristique
|
Nombre de cellules
|
Nombre caractéristique
|
Nombre de cellules
|
Nombre caractéristique
|
Nombre de cellules
|
Nombre caractéristique
|
Nombre de cellules
|
|
000
001
002
010
011
012
020
021
030
100
101
102
103
110
111
112
120
121
122
130
131
140
200
201
202
|
0.0
0.2
0.4
0.2
0.4
0.6
0.4
0.6
0.6
0.2
0.4
0.6
0.8
0.4
0.6
0.8
0.6
0.8
1.0
0.8
1.0
1.1
0.5
0.7
0.9
|
203
210
211
212
220
221
222
230
231
240
300
301
302
310
311
312
313
320
321
322
330
331
340
341
350
|
1.2
0.7
0.9
1.2
0.9
1.2
1.4
1.2
1.4
1.4
0.8
1.1
1.4
1.1
1.4
1.7
2.0
1.4
1.7
2.0
1.7
2.0
2.0
2.5
2.5
|
400
401
402
403
410
411
412
420
421
422
430
431
432
440
441
450
451
500
501
502
503
504
510
511
512
|
1.3
1.7
2.0
2.5
1.7
2.0
2.5
2.0
2.5
3.0
2.5
3.0
4.0
3.5
4.0
4.0
5.0
2.5
3.0
4.0
6.0
7.5
3.5
4.5
6.0
|
513
520
521
522
523
524
525
530
531
532
533
534
535
540
541
542
543
544
545
550
551
552
553
554
555
|
8.5
5.0
7.0
9.5
12.0
15.0
17.5
8.0
11.0
14.0
17.5
20.0
25.0
13.0
17.0
25.0
30.0
35.0
45.0
25.0
35.0
60.0
90.0
160.0
180.0
|
Annex i: Table of Mac Grady
Annex ii: Determination of amount of urine ingested from
farmers
Protocol
The simulation of this experimentation is done through spreading
the urine in the field in the same way a farmer would do. However, we used
gloves before handling urine. After we finished handling urine, we withdrew the
gloves and put them in sterilized bags to be analyzed. Then, the gloves were
rinsed in a fixed volume (100 mL) of ultra-pure water. The amount of nitrogen
was determined in this rinsed water. However, we must soak the gloves before
analysis to know if nitrogen is not on these gloves where the result may be of
use for control.
Assuming that all nitrogen proceeds from urine, we use the
equation of conservation of concentration to determine the amount of urine
ingested.
Where:
Vi= Volume of urine ingested (L),
Ci= Initial concentration of nitrogen contained in
stored urine (mg/L),
Vf= Volume of water where gloves soak (L),
Cf= Concentration of nitrogen of urine in rinsed
water (mg/L).
Annex iii: Calculation of Salmonella
infection risk from soil ingestion of C+TW treatment
Annex iv : Calculation of Salmonella
infection risk from lettuce consumption of C+TW treatment
 Annex v Calculation of Ascaris infection risk from soil ingestion of
C+TW treatment
Annex vi: Calculation of Ascaris infection risk from
lettuce consumption of C+TW treatment
Annex vii :Calculation of Salmonella
infection risk from soil ingestion of U+TW treatment
Annex viii : Calculation of Salmonella
infection risk from lettuce consumption of U+TW treatment
Annex ix : Calculation of Salmonella
infection risk from soil ingestion of GW treatment for 20 days
exposure
Annex x :Calculation of Salmonella
infection risk from soil ingestion of GW treatment for 275 days
exposure
Annex xi : Calculation of Salmonella
infection risk from lettuce consumption of GW treatment
Annex xii : Calculation of Salmonella
infection risk from soil ingestion of C+U+GW treatment for 20 days
exposure
Annex xiii : Calculation of Ascaris
infection risk from soil ingestion of C+U+GW treatment from 20 days
exposure
Annex xiv : Calculation of Salmonella
infection risk from soil ingestion of C+U+GW treatment for 275 days
exposure
Annex xv: Calculation of Ascaris infection risk
from soil ingestion of C+U+GW treatment from 275 days exposure
Annex xvi : Calculation of Salmonella
infection risk from lettuce consumption of C+U+GW treatment
Annex xvii : Calculation of Ascaris
infection risk from lettuce consumption of C+U+GW treatment
Annex xviii : Besoins en eau de la laitue
Annex xix : Initial amount of indicators and
pathogens in urine and greywater
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Matrix
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Indicators/Pathogens
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Urine
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Greywater
|
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E.coli
(log10CFU/100mL)
|
0
|
4.73#177;1.73
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Fecal coliforms
(log10CFU/100mL)
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5.12#177;0.71
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5.35#177;1.4
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Enterococci(log10CFU/100mL)
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4.00#177;0.41
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4.42#177;1.63
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Salmonella(log10
MPN/100mL)
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3.95 #177;2.27
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156.63#177;199.80
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Ascaris(eggs/L)
|
0
|
0
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Annex xx: Initial amount of indicators and pathogens in
compost and soil
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Matrix
|
|
Indicators/Pathogens
|
Compost
|
Soil
|
|
E.coli
(log10CFU/gDW)
|
4.21 #177;3.53
|
2.10 #177;2.20
|
|
Fecal coliforms
(log10CFU/gDW)
|
4.40 #177;3.55
|
0
|
|
Enterococci
(log10CFU/gDW)
|
5.82 #177;4.32
|
0
|
|
Salmonella
(log10MPN/g DW)
|
3.31
|
0
|
|
Ascaris(eggs/gDW)
|
27
|
0
|
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