Annexe 2 - Exemple de GPCR d'Aspergillus
parasiticus
Query: XP_024549350.1 (BcGPR3) Query ID: lcl|Query_65159
Length: 454
>hypothetical protein BDV34DRAFT_218387 [Aspergillus
parasiticus]
Sequence ID: KAB8199028.1 Length: 421
Range 1: 3 to 386
Score:251 bits(641), Expect:2e-77,
Method:Compositional matrix adjust.,
Identities:152/415(37%), Positives:225/415(54%),
Gaps:50/415(12%)
En rouge : D Y P I H C
En bleu : E L Y F P T V S A I
Aligné jaune : transmembrane de BcGPR3
Aligné vert : transmembrane de A. parasiticus
|
Query 4
LSQREIDILIIIERTTASISVMGTLALFGTFIFFKSFRTLSNT
|
|
|
|
IIFYASFANLFANVAAL
|
63
|
LS++++ + ER ++IS+ GT + +F+ +FR N ++FYAS+ NL N+A
|
+
Sbjct 3
LSEQQLYAISATERVC
|
|
|
|
|
SAISLTGTAIIVISFLSSSAFRKPI
|
NRLVFYASWGNLMTNIATV
|
62
|
Query 64 IGGSALSQ-
VNSPLCQFQGFLLEMFMQSDPWWSLAMATNVYLVFFYRFDAAQLKKLYPYY 122
I S + +NSPLCQ Q FL++ FM +D W+ AMA NVYL FFY++++ QL++L
|
Y
Sbjct 63
ISQSGIHMGLNSPLCQLQAFL
|
IQWFMPADALWTFAMACNVYLTFFYKYNSEQLRRLE
|
|
122
|
|
WKY
|
|
|
Query 123
ALICYGLPFIPAMVCLFVKNDKKGKIYGNATLWCWIDGPWAPLRIYSYYAPIWITILAAL
182
L CYGLPFIP++ F+K +GK+YG A W W LRI +Y P+W I+
L
Sbjct 123
VLFCYGLPFIPSLAYFFIKTKARGKVYGYAVTWDW
LRIAVFYGPVWFFIVLTL 175
Query 183
CIYVRVGIEIFRARNQLKEISHRTDPSLHTDAGGKSPLPLQGSITNASEATNKDYES--- 239
IY+R G I+ R QL+++ + D+ P E T + YE
Sbjct 176 AIYIRTGSIIWERRRQLRQLD NLDSADSYP
RYEGEPTAEHYEGFEM
221
Query 240 EHEQPIF--TGTR-
TTEIEVTHGSWENGTALSTNPTPRYPRASDVNHLSTPVKEPDSNYR 296
QP T TR TTEI VT + ++ + P A ++ + +P P Y
Sbjct 222 RDTQPPLEPTYTRPTTEIRVT
SEITRSQQGSCPTAEELCNPVSPFYNP---
YS 271
Query 297 VTI SAVP---
NTHTSNGRGGARRNASSLDKIKWAYTKVAMLFCISILITWV 344
VTI S +P H+ + RR S WAYTK AMLF
+++++TWV
Sbjct 272
VTIEGGFMSRQSSGLPLKAIKHSHSESWSRRRAMS DTSTAAWAYTKYAMLFFVALIVTWV
331
Query 345 PASVNRVYGLRFPDRPSFILNIGSAIVLPLQGFWNTVIYFTTSLGICRSVWADLR
399
P++VNR Y L +P+ +F LN S+ VLPLQGFWN++IY + S ++V+ D R
Sbjct 332
PSTVNRAYSLAYPNSYNFALNYVSSFVLPLQGFWNSIIYMSISWPAFKTVYRDFR 386
TMpred prediction output for : TMPRED.25814.8689.seq
|
Sequence: LSE...DFR length: 384
Prediction parameters: TM-helix length between 17 and 33
> STRONGLY prefered model: N-terminus inside
|
|
7
|
strong transmembrane helices, total score : 11789
|
|
#
|
from to
|
length
|
score
|
orientation
|
|
1
|
19
|
37
|
(19)
|
1764
|
i-o
|
|
2
|
44
|
62
|
(19)
|
956
|
o -i
|
3
|
84
|
106
|
(23)
|
1741
|
i-o
|
4
|
120
|
138
|
(19)
|
1894
|
o -i
|
5
|
158
|
180
|
(23)
|
2308
|
i-o
|
6
|
311
|
333
|
(23)
|
1862
|
o -i
|
|
|
7
|
352
|
376
|
(25)
|
1264
|
i-o
|
Annexe 3 - Exemple de GPCR de Penicillium expansum
Basé sur une identité de séquence
avec les séquences GPCR retrouvées chez A. flavus
En
rouge : D Y P I H C
Aligné vert : domaine transmembranaire
gprA // Fungal pheromone mating factor STE2
GPCR (97%, e-value = 3e-137)
>XP_016599889.1 Fungal pheromone mating factor STE2 GPCR
MAGTSSPTPSFDPYTQNVTFHLGDGTELLVPVQALDVFVMYNVRVCINYGCQFGASFTLLVILLLLTQSD
KRRSAVFILNGLALFLNSGRLLFQVIHFSTGFEKVYPYVSGDYSSVPWSAYAISIVAVILTTLVIGCIEA
SLVIQVHVVCSTLRRRYRHPLLVVSILVALVPIGFRSAWMVVNCNAIITLNYMSDIWWIESATNICLTIS
ICFFCVIFVTKLGFAIKQRRRLGVREFGPMKVILVMGCQTMVVPAIFSIIQYYITVPELASNVVTLVVIS
LPLSSIWAGAALEHSRRPGSQDNPHRRNLWRALVGGAESILSPTKDSPTSLSAMTAAQTLCYSDQTISKG
SHNSRDTDAFYGIAVEHDISINRVQRNNSIV
TMpred prediction output for : TMPRED.17363.7926.seq
Sequence: MAG...SIV length: 381
Prediction parameters: TM-helix length between 17 and 33
> STRONGLY prefered model: N-terminus outside
|
7 strong # from
|
transmembrane helices, total score : 13296
to length score orientation
|
|
1 51
|
69
|
(19)
|
2040 o-i
|
|
2 74
|
93
|
(20)
|
1116 i-o
|
|
3 120
|
136
|
(17)
|
2484 o-i
|
|
4 160
|
178
|
(19)
|
1847 i-o
|
|
5 205
|
226
|
(22)
|
2577 o-i
|
|
6 242
|
260
|
(19)
|
1664 i-o
|
|
7 273
|
292
|
(20)
|
1568 o-i
|
| 
