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Etude de l'effet immunomodulateur de la membrane lamellaire d'echinoccocus granulosus sur la production du NO au cours des MICIpar Sara Benazzouz Universite des sciences et de la technologie Houari Boumediane - Master en science de la nature et de la vie, speciality: Biotechnologie et pathologie moleculaire 2015 |
ConclusionConclusionAu regard global de notre étude, nous suggérons l'instauration d'un effet curatif exercé par l'extrait soluble de la membrane lamellaire hydatique dans le modèle de colite expérimentale induite par le DSS. En effet, il apparait que la ML exerce un effet immunomodulateur sur l'expression de la NOS2 qui se traduit par la diminution de la production du NO in vivo et in vitro au niveau des surnageants de culture des macrophages péritonéaux. D'autre part nous avons noté un effet bénéfique probable sur la protection de l'intégrité de la muqueuse colique. Nous avons dans cette optique, mis en évidence, le rôle immunomodulateur de cet extrait sur la production du NO ex vivo chez des patients atteints de MICI. En effet, les résultats obtenus à travers notre étude, sont en faveur de l'hypothèse hygiéniste et suggèrent un effet thérapeutique potentiel de la ML dans les MICI.
IL-10 1 Arginase i 1-1-1-1-1-1 1-1 N O52-444 t O5 Barrière épithéliale Lamina propria IL-4 Treg I TGF-j3 44 IL-10 Induction de la tolérance immunitaire aux bactéries commensales IL-4 IL-5 IL-13 el le li 'forrre 0 pn i Barrière épithéliale ONOO- Lamina propria IL-13 TNFa TGF-R1IL-1G IL-4 IL-5 L-13 IFN-y `IL-17e 1L-23 O2- Rupture de l'homéostasie intestinale TN Fat Lamina propria NOSJI ONOO- (y, Injection IP de la ML i -- 00 - 1- lme Barrière épithéliale tIArginase 1 NOS2 M211, 114 L IL-4 L-6 1
G F-13 1 IL-5 IL-101 IL-13 IL-4 "1.4IFN-y IL-131 1 Argi na se 11t TGF-131 I L-10 IL-23 X MI. TNFaI Treg NOS2 NOS2 TNFa IL-17a Figure 17 : Schéma proposé pour illustrer
les altérations induites par le DSS et l'effet Références bibliographiques Références bibliographiques :
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