ABSTRACT
Bacteriophages or phages are the viruses of bacteria and have
the ability to lyse them. This lytic action makes phages a therapeutic
alternative called phagotherapy against antibiotic therapy. The misuse of
antibiotic therapy is now causing a worldwide resurgence of bacterial
resistance to available anti-infectives. In Côte d'Ivoire, multiresistant
bacteria are reported in clinical and environmental studies. The unknown
phagotherapy of the professional medical profession and populations. Recent
studies have reported the presence of phages with therapeutic potential, but no
application is reported. The development of phage therapy requires complete
morphological and genomic characterization of phages with lytic potential. The
objective of determining the genomic profile of isolated bacteriophages in
Ivory Coast. Specifically, to analyze their genome by the technique of pulsed
field electrophoresis and enzymatic digestion. 5 phages including 2 lagoon
phages of the Ebrié Lagoon (p A5a, Ebrios) and 3 phages (M3, p M7, p
M11) isolated from the micromammals were included in this study. XbaI, XhoI and
XmnI enzymes have been used for digestion of genomes. The results obtained show
that the phages are double-stranded DNA phages. Pulsed field analysis shows
that phages p A5a, p M3, p M7, p M11 have a genome size greater than 164 Kpb.
The genomic diversity of phages is demonstrated by the restriction profiles of
pulsed field electrophoresis and enzymatic digestion. This study shows that
pulsed field gel electrophoresis (PFGE) is a more sensitive method than
enzymatic digestion for genomic characterization. On the other hand, the
enzymatic digestion of the phages is simple and fast and to determine the type
of phage genetic material.
Keywords: Bacteriophage, Genetic
diversity, PFGE, Enzymatic digestion, Ivory Coast.
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