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Development of organic soup from dried vegetables grown in Rwanda

( Télécharger le fichier original )
par UWAYO Désiré
Kigali University of Science and Technology (KIST) - Bachelor degree of Science in Food Science and Technology 2008
  

précédent sommaire

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REFERENCES

FRAZIER C.W., WESTHOFF C.D.(1988) Food microbiology, 4th edition,

Tata McGraw-Hill edition.

GARCIA-MATA M., BOSCH-BOSC N., PEREZ-RODRIGUEZ M.L (1995)

«Effects of cooking on nitrates in dehydrated soups». International journal of Food Science and Technology, Volume30, Issue1, page 45-48, February 1995.

HOUNSOME N., HOUNSOME B., THOMAS D., EDWARDS-JONES G. (2008)

«Plant metabolites and nutritional quality of vegetables» Journal of Food Science, Volume73, Issue4, page R48-R65, May 2008.

KENDALL P., DIPERSIO P., SOFOS J.(2006) Drying vegetables.

(www.ext.colostate.edu/PUBS/FOODNUT/09308.html)

MEILGAARD M., CIVILLE G.V., CARR B.T. (1999)

Sensory evaluation techniques, 3rd edition. CRC Press LLC

MITSURU NAKUMARA, KATHERINE D. KELLY(1968).

«Clostridium perfrigens in dehydrated soups and sauces».

Journal of Food Science, Volume33, Issue4, page 426-426, July 1968.

MICHELLE H., LOY R.S., RIVLIN M.D. (2001).Nutritional clinical care, Volume3,

Issue3, Pages 145-152. 25th December 2001

MONACO R.D., CAVELLA S., TORRIERI E., MASI P. (2007).  « Consumer acceptability of vegetable soups » Journal of sensory studies, Volume22, Issue1, page 81-98, February 2007

NIELSEN S.S (1998) Food analysis 2nd edition. Aspen publishers

ROLAND P.C. and LYON, H.D., (2000). Guidelines for Sensory Analysis in Food Products Development and Quality Control, 2nd Ed., an Aspen Publisher, Inc., USA

SHAKUNTALA M.N., SHADAKSHARASWAMY M.(2001)

FOODS : Facts and Principles, 2nd edition. New age international publishers.

TERRY P., TERRY J.B., and WALK A. (2001)

« Fruits and vegetable consumption in prevention of cancer»

Journal of international medicine, volume 250, Issue4, page280-290, October 2001

TOMOHIRO SEKI (1990), Laboratory manual for food analysis.

VIEIRA E. R. (1996) Elementary Food Science, 4th edition. Chapman & Hall

www.fao.org ( 24th July 2008 )

www.wikipedia.org ( 29th May 2008 )

APPENDICES

QUESTIONNAIRE FOR SUITABLE FOOD FORMULATION Date.............................

Instructions

Be sure that your hands are clean before starting and rinse your mouth before tasting and re-tasting, and then evaluate the products in front of you.

1°After eating on each of the 6 presented soups, complete the following tables by the symbol .

Soup1

 

Excellent

Very good

Good

Neither good nor bad

Bad

Very bad

Rejected

Appearance

 
 
 
 
 
 
 

Aroma

 
 
 
 
 
 
 

Colour

 
 
 
 
 
 
 

Taste

 
 
 
 
 
 
 

Texture

 
 
 
 
 
 
 

Overall acceptability

 
 
 
 
 
 
 

Soup2

 

Excellent

Very good

Good

Neither good nor bad

Bad

Very bad

Rejected

Appearance

 
 
 
 
 
 
 

Aroma

 
 
 
 
 
 
 

Colour

 
 
 
 
 
 
 

Taste

 
 
 
 
 
 
 

Texture

 
 
 
 
 
 
 

Overall acceptability

 
 
 
 
 
 
 

Soup3

 

Excellent

Very good

Good

Neither good nor bad

Bad

Very bad

Rejected

Appearance

 
 
 
 
 
 
 

Aroma

 
 
 
 
 
 
 

Colour

 
 
 
 
 
 
 

Taste

 
 
 
 
 
 
 

Texture

 
 
 
 
 
 
 

Overall acceptability

 
 
 
 
 
 
 

Soup4

 

Excellent

Very good

Good

Neither good nor bad

Bad

Very bad

Rejected

Appearance

 
 
 
 
 
 
 

Aroma

 
 
 
 
 
 
 

Colour

 
 
 
 
 
 
 

Taste

 
 
 
 
 
 
 

Texture

 
 
 
 
 
 
 

Overall acceptability

 
 
 
 
 
 
 

Soup5

 

Excellent

Very good

Good

Neither good nor bad

Bad

Very bad

Rejected

Appearance

 
 
 
 
 
 
 

Aroma

 
 
 
 
 
 
 

Colour

 
 
 
 
 
 
 

Taste

 
 
 
 
 
 
 

Texture

 
 
 
 
 
 
 

Overall acceptability

 
 
 
 
 
 
 

Soup6

 

Excellent

Very good

Good

Neither good nor bad

Bad

Very bad

Rejected

Appearance

 
 
 
 
 
 
 

Aroma

 
 
 
 
 
 
 

Colour

 
 
 
 
 
 
 

Taste

 
 
 
 
 
 
 

Texture

 
 
 
 
 
 
 

Overall acceptability

 
 
 
 
 
 
 

2. Comments: Please indicate what in particular you liked or disliked about this products (use words not sentence).

SOUP 1 SOUP 2 SOUP 3

Liked Disliked Liked Disliked Liked Disliked

.................. .................. .................. .................. .................. ..................

.................. .................. .................. .................. .................. ..................

.................. .................. .................. .................. .................. ..................

.................. .................. .................. .................. .................. ..................

SOUP 4 SOUP 5 SOUP 6

Liked Disliked Liked Disliked Liked Disliked

.................. .................. .................. .................. .................. ..................

.................. .................. .................. .................. .................. ..................

.................. .................. .................. .................. .................. ..................

.................. .................. .................. .................. .................. ..................

a. Do you want any of the characteristics to be changed ? Yes No

b. If Yes, suggest the changes................................................ .................................. .. .................................................................................................................... ..................................................................................................................... ......................................................................................................................

N.B: -Excellent > 80% -Neither good nor bad > 50% -Extremely bad(Rejected) < 20%

-Very good >70% -Bad > 40%

-Good > 60% -Very bad > 30%

QUESTIONNAIRE FOR SUITABLE COOKING TIME AND METHOD Date.....................

Instructions

Be sure that your hands are clean before starting and rinse your mouth before tasting and re-tasting, and then evaluate the products in front of you.

1°After eating on each of the 3 presented soups, complete the following tables by the symbol .

Soup1

 

Excellent

Very good

Good

Neither good nor bad

Bad

Very bad

Rejected

Appearance

 
 
 
 
 
 
 

Aroma

 
 
 
 
 
 
 

Colour

 
 
 
 
 
 
 

Taste

 
 
 
 
 
 
 

Texture

 
 
 
 
 
 
 

Overall acceptability

 
 
 
 
 
 
 

Soup2

 

Excellent

Very good

Good

Neither good nor bad

Bad

Very bad

Rejected

Appearance

 
 
 
 
 
 
 

Aroma

 
 
 
 
 
 
 

Colour

 
 
 
 
 
 
 

Taste

 
 
 
 
 
 
 

Texture

 
 
 
 
 
 
 

Overall acceptability

 
 
 
 
 
 
 

Soup3

 

Excellent

Very good

Good

Neither good nor bad

Bad

Very bad

Rejected

Appearance

 
 
 
 
 
 
 

Aroma

 
 
 
 
 
 
 

Colour

 
 
 
 
 
 
 

Taste

 
 
 
 
 
 
 

Texture

 
 
 
 
 
 
 

Overall acceptability

 
 
 
 
 
 
 

2. Comments: Please indicate what in particular you liked or disliked about this products (use words not sentence).

SOUP 1 SOUP 2 SOUP 3

Liked Disliked Liked Disliked Liked Disliked

.................. .................. .................. .................. .................. ................... .................. .................. .................. .................. .................. ..................

.................. .................. .................. .................. .................. ..................

N.B: -Excellent > 80% -Neither good nor bad > 50% -Extremely bad(Rejected) < 20%

-Very good >70% -Bad > 40%

-Good > 60% -Very bad > 30%

Thank you for evaluating our product!!!

QUESTIONNAIRE FOR CONSUMER ACCEPTABILITY CHECK Date:..................

1. Before opening

Dislike very much

Dislike moderately

Neither like nor

dislike

Like moderately

Like very much

1.1. Without opening, look at the product in the package, evaluate it and indicate your consideration basing on the following:

Appearance 5 4 3 2 1

Colour 5 4 3 2 1

Texture 5 4 3 2 1

1.2. Please, observe carefully the packaging and the labeling and then indicate how much you liked or disliked them. Show your score by circling one of the numbers below that matches the best with your overall consideration.

5

4

3

2

1

1.3. Please answer these question in accordance to you

a) Are the packaging and the labeling attractive enough? Yes No

b) Can you suggest changes, that can be made in order to make them more attractive!

.....................................................................................................................

......................................................................................................................

.....................................................................................................................

c) Are the information given on the label sufficient? Yes No

d) Is there any thing, you think is missing? ......................................................................

.......................................................................................................................

......................................................................................................................

2. After opening

2.1. Open the package and try to fill how the product smells and then indicate how much you appreciated it. Show your score by circling one of the numbers below that matches the best with your overall consideration.

5

4

3

2

1

3. After preparing

3.1. Prepare the soup, following the instructions given on the label.

Please observe, eat and taste this product and then, indicate how much you liked or disliked it. Show your score by circling one of the numbers below that matches the best with your overall consideration.

5

4

3

2

1

3.2. Comments: Please indicate what in particular you liked or disliked about this product (use words not sentences).

Liked Disliked

....................................... ...........................................

....................................... ...........................................

....................................... ...........................................

3.3. Assessment of particular characteristics

Please re-taste the product as needed and indicate how much you liked or disliked the following. Show your score by circling one of the numbers below that matches the best with your overall consideration.

Dislike very much

Dislike moderately

Neither like nor

dislike

Like moderately

Like very much

Appearance 5 4 3 2 1

Aroma 5 4 3 2 1

Colour 5 4 3 2 1

Taste 5 4 3 2 1

Texture 5 4 3 2 1

Thank you very much for evaluating our product!!!

DETERMINATION OF TOTAL REDUCING SUGAR BY LANE-EYNON METHOD

1. EQUIPMENTS AND REGENTS NEEDED

- Laboratory glassware

- Electronic balance

- Bunsen burner

- Fehling solution 1 ( cooper sulphate solution )

- Fehling solution 2 (alkaline tartrate solution )

- Standard invert sugar solution ( for standardization of Fehling so in.1)

- Methylene blue solution (1% in water)

2. SAMPLE PREPARATION

Dissolve 5g of sample with water and dilute to 100ml in a volumetric flask.

NOTE: the sample sugar solution is prepared so that 100ml of the solution

Contain 200 ~ 250mg of reducing sugar

3. PROCEDURE

3.1. standardization of Fehling solution

- Pipette each 5ml of Fehling solution. 1& 2

- Add 19ml of standard invert sugar solution from burette , and mix well

- Boil 4min ( keeping to avoid bumping)

- Add 4 drops of methylene blue

- Without removing the flame, complete the titration with standard invert sugar solution, until the indicator is decolorized. Record the volume required

* the titration should be finished

10ml, of mixed Fehling solution should require 20.36ml of standard invert sugar therefore

Factor of Fehling solution. ( f ) = ml of standard invert sugar required / 20.36

NOTE: titration volume of standard invert sugar should be 20.36 #177; 0.5ml if difference is more than that adjust concentration of Fehling solution 1 by addition of copper sulphate or water

3.2. preliminary titration

- Pipette each 5ml of Fehling solution.1&2

- Add 15ml of sample sugar solution from burette and mix well

- Heat the mixture to boil ( keeping to avoid bumping )

- Add 4 drops of methylene blue if blue color is disappeared already, dilute sample solution and try again from 1

- Without removing the flame, complete the titration with sample sugar solution, until the indicator is decolorized. Record the volume required

3.3. final titration

- Pipette each 5ml of Fehling solution. 1 & 2

- Add the sample solution of which volume is 1ml less than the volume required in the preliminary titration.

- Boil for 2min ( keeping to avoid bumping )

- Add 4 drops of metylene blue

- Without removing the flame complete the titration until the indicator is decolorized with sugar solution.

* the titration should be finished within 3min.

4. CALCULATION

Total reducing sugar content ( %) =

a = Lane-Eynon factor as mg of reducing sugar corresponding to the milliliters of sample sugar

solution required from table

b = ml of the sample sugar solution required

f = Factor of Fehling solution 1

s = Weight of the sample taken (g )

v = Volume of the sample solution prepared

DETERMINATION OF THE FAT CONTENT BY THE SOXHLET METHOD

1. EQUIPMENTS AND REAGENT NEEDED

- Laboratory electron balance

- Laboratory flasks

- Extraction thimble

- Soxhlet extraction apparatus

- Ethyl ether

2. SAMPLE PREPARTION

The sample that will be analyzed by the Soxhlet method must be well dried and grinded so as to pass a N°30 sieve. It must be stored in an air-tight bottle if it is not used immediately.

3. PROCEDURE

- Weigh accurately about 5g of sample ( W1)

- Transfer to an extraction thimble and stopper with defatted cotton wool

- Dry at 105°C - 110°C for one hour

- Place the thimble in the Soxhlet extraction apparatus

- Put ethyl ether up to 2/3 of flask

- Set up the apparatus and start extraction. (the temperature has to be controlled so that 80

condensed drops fall down on the thimble per minute, and keep this for 8 - 16hours).

- Evaporate the used ether, so as to remain with lipids only

- Weigh the fat extracted ( W2 )

- Calculate the percentage fat of the food by the formula:

% Fats =

DETERMINATION OF PROTEIN CONTENT BY THE KJELDAHL METHOD

4. EQUIPMENTS AND REAGENT NEEDED

- Laboratory glassware apparatus

- Electron balance

- Concentrated H2SO4

- Potassium sulfate

- Copper sulfate

- 45% NaOH solution

- 4% H3BO3 Solution

- Mixed indicator

- Standard 0.02N- HCl solution

5. SAMPLE PREPARTION

The sample that will be analyzed by the Kjeldahl method must be well dried and grinded so as to pass a N°30 sieve. It must be stored in an air-tight bottle if it is not used immediately.

6. PRINCIPLE AND PROCEDURE

The food is digested first, by means of heating it into a digestion flask, in presence of concentrated sulfuric acid (which digest the food), sodium or potassium sulfate (to speed up the reaction by rising the boiling point) and copper sulfate (as catalyst). This digestion converts nitrogen in the food into ammonia in the form of ammonium (NH4+) which binds to the sulfate ions (SO42-), and produces gases such as CO2, H2O.

H2SO4 / Heat

N (food) Catalysts (NH4) 2SO4

After digestion has been completed, the solution in the digestion flask must be made alkaline by addition of sodium hydroxide, which converts the ammonium sulfate into ammonia gas.

(NH4) 2SO4 +2NaOH 2NH3 + 2H2O + Na2 SO4

The ammonia gas produced is received into another flask, containing an excess of boric acid. The low pH of the solution in the receiving flask converts ammonia gas into the ammonium ion, and simultaneously converts the boric acid to borate ion:

NH3 +H3BO3 NH4+ + H2BO3-

The nitrogen content is then estimated by titration of the ammonium borate formed, by standard sulfuric or hydrochloric acid, using a suitable indicator to determine the end-point of the reaction. The concentration of hydrogen ions (in moles) required to reach the end-point, is equivalent to the concentration of nitrogen that was in the original food.

H2BO3- + H+ H3BO3

The following is the equation that can be used to determine the nitrogen concentration of a sample that weighs m grams, using xM HCl acid solution for the titration.

% Nitrogen =

Where x = Molar concentration of the acid used for titration

Vs= Titration volume for the sample

Vb= Titration volume for the blank

m = Mass of the original sample

Once the nitrogen content has been determined, it is converted to a protein content using the appropriate conversion factor F (usually 6.25 is used).

% Proteins = F % Nitrogen

DETERMINATION OF VITAMIN C CONTENT BY INDOPHENOL SOLUTION

1. EQUIPMENTS AND REAGENT NEEDED

- Laboratory glassware apparatus

- Electron balance

- 10% TCA solution

- Standard ascorbic acid solution (1mg/ml)

- Standard Indophenol solution

2. PRINCIPLE AND PROCEDURE

The determination of vitamin C is based on the fact that it is titratable by Indophenol in presence of Trichloroacetic Acid (TCA) solution.

The procedure of the experiment is briefly described below.

- Standardization of Indophenol solution

- Weigh a sample, grind it, and mixing the ground sample with TCA in a flask

- Titrating with Indophenol solution

- Titrating also TCA without sample (for blank)

- Calculate the % Vit C, by the formulae

% Vit C (mg/100g) = (A - B) C 100/10 1/S 100

Where A = Volume (in ml) of Indophenol solution used for sample

B = Volume (in ml) of Indophenol solution used for blank

C = Mass (in mg) of ascorbic acid equivalent to 1.0 ml of standard Indophenol

solution

S = Weight of the sample taken.

TABLES OF RESULTS FOR CONSUMMER ACCEPTABILITY CHECK ( BEFORE OPENING)

 

Score per sensory attributes

 

Panelist

Appearance

Aroma

Colour

Texture

Total

 

1

4

4

5

5

18

 

2

4

4

5

4

17

 

3

5

4

5

4

18

 

4

4

5

4

4

17

 

5

4

4

5

4

17

 

6

3

4

3

3

13

 

7

5

5

5

5

20

 

8

5

4

4

5

18

 

9

5

4

4

4

17

 

10

4

5

5

4

18

 

11

4

4

3

4

15

 

12

5

4

5

4

18

 

13

4

4

5

5

18

 

14

4

5

4

3

16

 

15

4

3

5

5

17

Total

 

64

63

67

63

257

Average

 

4.26666667

4.2

4.466667

4.2

 

= (257)2/ 4*15 = 66049/60 = 1100.817

SSS = () / N = (642+632+672+632) / 15 = 0.716667

TSS = 1125 - 1100.817 = 24.18333

ESS = 24.18333 - 0.716667 = 23.46667

DfT = ( n*N ) - 1 = (4*15) - 1 = 60 - 1 = 59

DfS = n - 1 = 4 - 1 = 3

DfE = DfT - DfS = 59 - 3 = 56

MST = = = 0.409887

MSS = = = 0.238889

EMS = = = 0.419048

Fcalc = = = 0.570076

Ftab at 5% = 2.774

Ftab at 1% = 4.163

TABLES OF RESULTS FOR CONSUMMER ACCEPTABILITY CHECK ( AFTER OPENING)

 

Score per sensory attributes

 

Panelist

Appearance

Aroma

Colour

Taste

Texture

Over.accept

total

 

1

5

4

5

5

5

3

27

 

2

4

5

4

4

3

4

24

 

3

5

4

5

5

4

5

28

 

4

4

5

4

3

4

3

23

 

5

5

4

5

4

3

4

25

 

6

4

5

5

5

5

5

29

 

7

5

5

5

5

5

5

30

 

8

5

4

4

4

5

4

26

 

9

5

5

4

3

5

4

26

 

10

4

5

4

5

4

4

26

 

11

4

5

4

4

5

4

26

 

12

5

5

5

4

4

4

27

 

13

5

4

5

3

5

5

27

 

14

4

4

5

5

3

5

26

 

15

4

3

5

4

5

4

25

Total

 

68

67

69

63

65

63

395

Average

 

4.533333333

4.466667

4.6

4.2

4.333333

4.2

 

= (396)2 / 15*6 = 156025 / 90 = 1733.611

SSS = [ () / N ] - CF = [ ( 682+672+692+632+652+632) / 15 ] - 1733.611 = 2.188889

TSS = 1773 - 1733.611 = 39.38889

ESS = 39.38889 - 2.188889 = 37.2

DfT = ( n*N ) - 1 = (15*6) - 1 = 89

DfS = n - 1 = 6 - 1 = 5

DfE = DfT - DfS = 89 - 5 = 84

MST = = = 0.442572

MSS = = = 0.437778

EMS = = = 0.442857

Fcalc = = = 0.98853

Ftab at 5% = 2.3368

Ftab at 1% = 3.273

PRICING MECHANISM FOR THE DEVELOPED SOUP

Object

Quantity(Kg)

U.Price (Frw)

Total (Frw)

Carrot

5

250

1250

Celery

1.5

150

225

Garlic

1

1200

1200

Leek

1

150

150

Onion

3

250

750

Pepper

1

400

400

Potatoes

3

100

300

Spinach

3

400

1200

Tomatoes

7.4

100

740

Salt

0.4

200

80

 TOTAL

6295 Frws

1. Cost of ingredients

2. Other expenses

- Transport 4000 Frw

- Energy 4000 Frw

- Labor 6000 Frw

- Tools and machinery 5000 Frw

TOTAL 25 295 Frw

?Reasonable benefit margin of 15% = = 3794.25 Frw

?Total revenue must be of 25295 Frw + 3764.25 Frw = 29089.25 Frw

Since 18 packets were produced, it was deduced that the price of one packet of flavoured dry vegetable soup would be 1616.069 Frw.

LABEL USED

1. RECTO OF THE PACKAGE

INGREDIENTS

Carrot Carotte

Potato P.de Terre

Tomato Tomate

Onion Ognon

Garlic Ail

Leek Poireaux

Spinach Epinard

Celery Céleri

Salt Sel de cuisine

Red pepper Piment

Manufact : 05 / 08 / 2008

Best before end ...........

Net Weight : 60 g Store in a cool & dry place

Manufactured at KIST by UWAYO Désiré, 4th year, Food science and technology

(Tel. N° 08 76 82 76)

Made in Rwanda Lot N° .........

INSTRUCTIONS FOR USE

- Put 1 ltr (3 small metal tea cups) of water in a cooking pan

- Add the content of the package

- Add about 10 ml

(3 soup spoons) of cooking oil

- Add a pink of salt to taste

- Boil for 15 - 20 minutes

- Keep stirring while boiling

- Serve hot

MODE D`EMPLOI

-Mettez 1 ltr (3 petits gobelets en métal) d`eau dans un casserole

-Ajoutez le contenu d`un paquet

-Ajoutez 10 ml

(3 cuillères à soupe) d`huile

-Mettez un peu de sel selon votre goût

- Faites bouillir pendant 15-20 minutes tout en remuant.

-Servez chaud

2. VERSO OF THE PACKAGE

NUTRITIONAL VALUE(per100g)

- 4.98% moisture content

- 95.02% total solid matter

- 13.06% crude protein

- 3.17% lipids

- 8.44% ash & minerals

- 1.66% vitamin C

- 68.69% total soluble sugars

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